Hotspot

petN-psbM

LSC · 28710-29923 · 1214 bp

Back to Lasianthus

Pi 0.00387

MarkerSeek score 61.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1214
variable_sites: 7
pi: 0.00387
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACGGCCTTTCTACT	50.0	47.6	58.0	58.2	1294-1303 bp	1.0	71.5
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACGGCCTTTCTACT	50.0	47.6	58.0	58.2	1292-1301 bp	1.0	71.5
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	AGTTCCTACGGCCTTTCTACT	55.0	47.6	57.9	58.2	1291-1300 bp	1.0	70.9
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACGGCCTTTCTACT	50.0	47.6	58.2	58.2	1346-1355 bp	1.0	70.3
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	GTTCCTACGGCCTTTCTACT	50.0	50.0	58.0	56.7	1291-1300 bp	1.0	70.2