Hotspot

rpoB-trnC-GCA

LSC · 26822-27617 · 796 bp

Back to Lasianthus

Pi 0.00251

MarkerSeek score 61.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 3
pi: 0.00251
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCAACAAACCCCGGTCAATA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	955-1074 bp	1.0	79.5
rpoB-trnC-GCA_p2	CCAACAAACCCCGGTCAATA	TCTTGTGTTGATCAGGCGAC	50.0	50.0	58.1	58.2	977-1096 bp	1.0	78.5
rpoB-trnC-GCA_p3	AACCCCGGTCAATAAACCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	949-1068 bp	1.0	78.5
rpoB-trnC-GCA_p4	AGTTCTTCCAACAAACCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.3	58.1	962-1081 bp	1.0	77.7
rpoB-trnC-GCA_p5	AACCCCGGTCAATAAACCTG	TCTTGTGTTGATCAGGCGAC	50.0	50.0	57.8	58.2	971-1090 bp	1.0	77.5