Hotspot

atpH-atpI

LSC · 13571-14381 · 811 bp

Back to Lasianthus

Pi 0.00329

MarkerSeek score 62.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 4
pi: 0.00329
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCC	45.0	45.0	58.1	58.1	897-902 bp	1.0	79.2
atpH-atpI_p2	AACAGAAGCGGCAGAAATCA	TAGCCGCCGCTTATATAGGT	45.0	50.0	58.1	58.2	885-890 bp	1.0	79.1
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTAGCCGCC	50.0	45.0	57.8	58.1	907-912 bp	1.0	78.7
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	TAGCCGCCGCTTATATAGGT	50.0	50.0	57.8	58.2	895-900 bp	1.0	78.5
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCC	50.0	45.0	57.8	58.1	914-919 bp	1.0	78.3