Hotspot

rps16-trnQ-UUG

LSC · 5819-6709 · 891 bp

Back to Lasianthus

Pi 0.00561

MarkerSeek score 67.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 891
variable_sites: 6
pi: 0.00561
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	949-982 bp	1.0	82.8
rps16-trnQ-UUG_p2	AGACAGCGGTGGATAGAAGA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	996-1029 bp	1.0	82.7
rps16-trnQ-UUG_p3	CGAAATAGACAGCGGTGGAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1002-1035 bp	1.0	82.1
rps16-trnQ-UUG_p4	AGACAGCGAAATAGACAGCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1023 bp	0.333333	52.0
rps16-trnQ-UUG_p5	AGACAGCGAAATAGACAGCG	TAAAAATCGAAGTGGGGCGT	50.0	45.0	58.1	57.8	1083 bp	0.333333	51.1