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rps8

LSC · 80535-80939 · 405 bp

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Pi 0.00741

MarkerSeek score 58.90

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 3
pi: 0.00741
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	GATATTAGACAACCCGCCCC	TGTGTATCATGCATATGCCT	55.0	40.0	57.8	54.6	590-591 bp	1.0	51.5
rps8_p2	ATATTAGACAACCCGCCCCT	TGTGTATCATGCATATGCCT	50.0	40.0	58.2	54.6	589-590 bp	1.0	51.5
rps8_p3	CGATATTAGACAACCCGCCC	TGTGTATCATGCATATGCCT	55.0	40.0	58.2	54.6	591-592 bp	1.0	51.3
rps8_p4	AAGTTTCGGACCCAATTCGT	TGTGTATCATGCATATGCCT	45.0	40.0	57.7	54.6	545-546 bp	1.0	50.9
rps8_p5	GATATTAGACAACCCGCCCC	TGTGTATCATGCATATGCCTT	55.0	38.1	57.8	55.4	590-591 bp	1.0	50.3