Hotspot

rpoA

LSC · 78292-79353 · 1062 bp

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Pi 0.00188

MarkerSeek score 59.80

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	ACGACCTGTGTATCTAGGGA	TGTAGACCTCCGAAAAAGCG	50.0	50.0	56.9	57.9	1256-1262 bp	1.0	70.6
rpoA_p2	CACGACCTGTGTATCTAGGG	TGTAGACCTCCGAAAAAGCG	55.0	50.0	56.8	57.9	1257-1263 bp	1.0	70.3
rpoA_p3	ACGACCTGTGTATCTAGGGA	CGAAAAAGCGACGTGTGTAG	50.0	50.0	56.9	57.8	1246-1252 bp	1.0	69.7
rpoA_p4	CACGACCTGTGTATCTAGGG	CGAAAAAGCGACGTGTGTAG	55.0	50.0	56.8	57.8	1247-1253 bp	1.0	69.3
rpoA_p5	CACGACCTGTGTATCTAGGGA	TGTAGACCTCCGAAAAAGCG	52.4	50.0	58.3	57.9	1257-1263 bp	1.0	68.7