Hotspot

ndhK

LSC · 50216-50893 · 678 bp

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Pi 0.00295

MarkerSeek score 60.40

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 678
variable_sites: 2
pi: 0.00295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TTTGCGGATTGACCCACTAC	AAAAGGGGCATTGGAATGGT	50.0	45.0	58.2	58.0	801 bp	1.0	78.4
ndhK_p2	TCTCTCCCGCATTGTTTTGT	AAAAGGGGCATTGGAATGGT	45.0	45.0	57.7	58.0	856 bp	1.0	77.7
ndhK_p3	TTTTTGCGGATTGACCCACT	AAAAGGGGCATTGGAATGGT	45.0	45.0	58.3	58.0	803 bp	1.0	77.4
ndhK_p4	TTTGCGGATTGACCCACTAC	CGAAAAGGGGCATTGGAATG	50.0	50.0	58.2	57.7	803 bp	1.0	76.3
ndhK_p5	TCTCTCCCGCATTGTTTTGT	CGAAAAGGGGCATTGGAATG	45.0	50.0	57.7	57.7	858 bp	1.0	75.6