Hotspot

ndhH

SSC · 124639-125820 · 1182 bp

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Pi 0.00169

MarkerSeek score 59.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 2
pi: 0.00169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AGGGACAAGCATCCATATGA	ACCTCTTTTCGCTTTCAGCA	45.0	45.0	56.0	58.0	1330 bp	1.0	63.6
ndhH_p2	AGGGACAAGCATCCATATGATC	ACCTCTTTTCGCTTTCAGCA	45.5	45.0	57.7	58.0	1330 bp	1.0	61.5
ndhH_p3	GGGACAAGCATCCATATGATCT	ACCTCTTTTCGCTTTCAGCA	45.5	45.0	57.7	58.0	1329 bp	1.0	61.5
ndhH_p4	AGGGACAAGCATCCATATGAT	ACCTCTTTTCGCTTTCAGCA	42.9	45.0	56.5	58.0	1330 bp	1.0	60.1
ndhH_p5	GGGACAAGCATCCATATGATC	ACCTCTTTTCGCTTTCAGCA	47.6	45.0	56.2	58.0	1329 bp	1.0	57.7