Hotspot

matK

LSC · 1792-3336 · 1545 bp

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Pi 0.00259

MarkerSeek score 60.10

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1545
variable_sites: 4
pi: 0.00259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	CAAAAAGTGGAATACTCGGTTGA	ACCCCCAAAATCCTCTACCT	39.1	50.0	57.3	57.6	1684 bp	1.0	47.7
matK_p2	TCAAAAAGTGGAATACTCGGTTG	ACCCCCAAAATCCTCTACCT	39.1	50.0	57.3	57.6	1685 bp	1.0	47.7
matK_p3	AAAGTGGAATACTCGGTTGA	ACCCCCAAAATCCTCTACCT	40.0	50.0	54.3	57.6	1681 bp	1.0	47.6
matK_p4	AAAAGTGGAATACTCGGTTGA	ACCCCCAAAATCCTCTACCT	38.1	50.0	55.2	57.6	1682 bp	1.0	46.4
matK_p5	TCAAAAAGTGGAATACTCGGT	ACCCCCAAAATCCTCTACCT	38.1	50.0	55.2	57.6	1685 bp	1.0	46.4