Hotspot

ndhF-rpl32

SSC · 114933-116120 · 1188 bp

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Pi 0.00085

MarkerSeek score 60.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1188
variable_sites: 1
pi: 0.00085
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	GCTGCCCAATATCCCTTACC	50.0	55.0	58.0	58.1	1365-1369 bp	1.0	79.8
ndhF-rpl32_p2	GTTGCCGACGGAAAAAGAAG	GGCTGCCCAATATCCCTTAC	50.0	55.0	58.0	58.1	1366-1370 bp	1.0	79.8
ndhF-rpl32_p3	TGCCGACGGAAAAAGAAGAA	GCTGCCCAATATCCCTTACC	45.0	55.0	58.0	58.1	1363-1367 bp	1.0	79.7
ndhF-rpl32_p4	TGCCGACGGAAAAAGAAGAA	GGCTGCCCAATATCCCTTAC	45.0	55.0	58.0	58.1	1364-1368 bp	1.0	79.7
ndhF-rpl32_p5	TTGCCGACGGAAAAAGAAGA	GCTGCCCAATATCCCTTACC	45.0	55.0	58.0	58.1	1364-1368 bp	1.0	79.7