Hotspot

accD-psaI

LSC · 59332-59765 · 434 bp

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Pi 0.00461

MarkerSeek score 43.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 2
pi: 0.00461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAGCCGTTCAAACAGGCATA	GGATAATGGCCCAGCAGAAA	45.0	50.0	58.1	57.9	1580-1582 bp	1.0	78.9
accD-psaI_p2	AAGCCGTTCAAACAGGCATA	TTTTCGCGACCCCGTTATAG	45.0	50.0	58.1	58.1	1552-1554 bp	1.0	78.8
accD-psaI_p3	GATCCGGATACTTGGGATGC	GGATAATGGCCCAGCAGAAA	55.0	50.0	57.9	57.9	1704-1706 bp	1.0	78.8
accD-psaI_p4	AAGCCGTTCAAACAGGCATA	AAAGGATAATGGCCCAGCAG	45.0	50.0	58.1	57.9	1583-1585 bp	1.0	78.8
accD-psaI_p5	GATCCGGATACTTGGGATGC	TTTTCGCGACCCCGTTATAG	55.0	50.0	57.9	58.1	1676-1678 bp	1.0	78.8