Hotspot

trnT-GGU-psbD

LSC · 32155-33408 · 1254 bp

Back to Laserpitium

Pi 0.00159

MarkerSeek score 59.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1254
variable_sites: 2
pi: 0.00159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AAGGGCCCTTTTAACTCAGC	AGACCGGACCATCCTACAAA	50.0	50.0	58.1	58.1	1447-1452 bp	1.0	79.1
trnT-GGU-psbD_p2	GAATTGAAGCAAAAGGGCCC	AGACCGGACCATCCTACAAA	50.0	50.0	57.9	58.1	1459-1464 bp	1.0	78.9
trnT-GGU-psbD_p3	GGAATTGAAGCAAAAGGGCC	AGACCGGACCATCCTACAAA	50.0	50.0	57.9	58.1	1460-1465 bp	1.0	78.9
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	AGACCGGACCATCCTACAAA	50.0	50.0	57.9	58.1	1443-1448 bp	1.0	78.9
trnT-GGU-psbD_p5	AAGGGCCCTTTTAACTCAGC	GAGCAATAGACCGGACCATC	50.0	55.0	58.1	57.8	1454-1459 bp	1.0	78.3