Hotspot

petN-psbM

LSC · 29019-30165 · 1147 bp

Back to Laserpitium

Pi 0.00351

MarkerSeek score 61.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1147
variable_sites: 4
pi: 0.00351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1313-1321 bp	1.0	68.3
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1252-1260 bp	1.0	66.7
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	58.2	57.2	1292-1300 bp	1.0	65.3
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1313-1321 bp	1.0	64.1
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	57.6	57.2	1231-1239 bp	1.0	63.7