Hotspot

rpoB-trnC-GCA

LSC · 26984-28205 · 1222 bp

Back to Laserpitium

Pi 0.00245

MarkerSeek score 60.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1222
variable_sites: 3
pi: 0.00245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATCTCGGA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1268 bp	1.0	78.3
rpoB-trnC-GCA_p2	TCCCTCATTTCCATCTCGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1308 bp	1.0	78.1
rpoB-trnC-GCA_p3	AGCCCTGATCCATGAACCTA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1335 bp	1.0	78.1
rpoB-trnC-GCA_p4	AGCCCTGATCCATGAACCTA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1375 bp	1.0	77.9
rpoB-trnC-GCA_p5	GCCCTGATCCATGAACCTAC	CTTACCACTCGGCCATATCG	55.0	55.0	57.7	57.9	1334 bp	1.0	77.6