Hotspot

ycf2

Genome · 78147-78733 · 587 bp

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Pi 0.00996
MarkerSeek score 44.10
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
Genome
length_bp
587
variable_sites
19
pi
0.00996
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 TTTCCATGATATATTCTCATCCA AGCTCTCTTCACTCCAGAGG 30.4 55.0 52.8 58.1 729-734 bp 1.0 42.2
ycf2_p2 TTTCCATGATATATTCTCATCCA CTCTCTTCACTCCAGAGGCT 30.4 55.0 52.8 58.1 727-732 bp 1.0 42.2
ycf2_p3 TTTCCATGATATATTCTCATCCA TCTCTTCACTCCAGAGGCTC 30.4 55.0 52.8 58.2 726-731 bp 1.0 42.2
ycf2_p4 TCCATGATATATTCTCATCCATT AGCTCTCTTCACTCCAGAGG 30.4 55.0 52.5 58.1 727-732 bp 1.0 42.2
ycf2_p5 TCCATGATATATTCTCATCCATT CTCTCTTCACTCCAGAGGCT 30.4 55.0 52.5 58.1 725-730 bp 1.0 42.2