Hotspot

petB

Genome · 23943-25384 · 1442 bp

Pi 0.00323

MarkerSeek score 52.40

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: Genome
length_bp: 1442
variable_sites: 18
pi: 0.00323
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AGGGATCCAAACTCAGACCA	CCGTTGTTACTCGGAACAAG	50.0	50.0	58.0	56.8	1599 bp	1.0	70.9
petB_p2	AGGGATCCAAACTCAGACCA	CCGTTGTTACTCGGAACAAGA	50.0	47.6	58.0	58.3	1599 bp	1.0	70.6
petB_p3	AGGGATCCAAACTCAGACCA	ACCGTTGTTACTCGGAACAAG	50.0	47.6	58.0	58.5	1600 bp	1.0	68.5
petB_p4	GAGGGATCCAAACTCAGACC	CCGTTGTTACTCGGAACAAG	55.0	50.0	57.3	56.8	1600 bp	1.0	65.6
petB_p5	GAGGGATCCAAACTCAGACC	CCGTTGTTACTCGGAACAAGA	55.0	47.6	57.3	58.3	1600 bp	1.0	65.4