Hotspot

ndhK

Genome · 35801-36600 · 800 bp

Pi 0.0035

MarkerSeek score 55.40

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: Genome
length_bp: 800
variable_sites: 8
pi: 0.0035
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TCGCTCCAGTTCTTGTTGTT	TCCGAACCGAAATGGAATGT	45.0	45.0	57.7	57.5	950-957 bp	1.0	75.2
ndhK_p2	TCGCTCCAGTTCTTGTTGTT	CTATTCATCCCGTCCGAACC	45.0	55.0	57.7	57.8	962-969 bp	0.416667	53.9
ndhK_p3	TTCGCTCCAGTTCTTGTTGT	CTATTCATCCCGTCCGAACC	45.0	55.0	57.7	57.8	963-970 bp	0.416667	53.9
ndhK_p4	TGTTCGCTCCAGTTCTTGTT	CTATTCATCCCGTCCGAACC	45.0	55.0	57.7	57.8	965-972 bp	0.416667	53.8
ndhK_p5	ATGTTCGCTCCAGTTCTTGT	CTATTCATCCCGTCCGAACC	45.0	55.0	57.4	57.8	966-973 bp	0.416667	52.0