Hotspot

psbE-petL

Genome · 115937-117188 · 1252 bp

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Pi 0.00367
MarkerSeek score 58.20
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
Genome
length_bp
1252
variable_sites
15
pi
0.00367
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AATGTCAGCAAAGGAGCGTT GCGTGGAAGGACTGAATGAA 45.0 50.0 58.4 58.2 1325-1334 bp 1.0 76.8
psbE-petL_p2 TAATGTCAGCAAAGGAGCGT GCGTGGAAGGACTGAATGAA 45.0 50.0 57.5 58.2 1326-1335 bp 1.0 76.2
psbE-petL_p3 AATGTCAGCAAAGGAGCGTT TAGGCGTGGAAGGACTGAAT 45.0 50.0 58.4 58.4 1328-1337 bp 1.0 74.9
psbE-petL_p4 AATGTCAGCAAAGGAGCGTT ATAGTAGGCGTGGAAGGACT 45.0 50.0 58.4 57.5 1332-1341 bp 1.0 74.7
psbE-petL_p5 TAATGTCAGCAAAGGAGCGT TAGGCGTGGAAGGACTGAAT 45.0 50.0 57.5 58.4 1329-1338 bp 1.0 74.3