Hotspot

petA-psbJ

Genome · 114141-115170 · 1030 bp

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Pi 0.00628
MarkerSeek score 42.50
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
Genome
length_bp
1030
variable_sites
17
pi
0.00628
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGGACCGGAACTTCTTGTTT GGCAATGCAGTTCATCCAAC 50.0 50.0 58.0 58.0 1672-1674 bp 1.0 80.9
petA-psbJ_p2 TCCCCATCCTTTCACCAGAT GGCAATGCAGTTCATCCAAC 50.0 50.0 58.0 58.0 1916-1918 bp 1.0 80.8
petA-psbJ_p3 CCTAATGTGGGTGGATTCGG GGCAATGCAGTTCATCCAAC 55.0 50.0 58.0 58.0 1608-1610 bp 1.0 80.8
petA-psbJ_p4 TCCCCATCCTTTCACCAGAT AGTAACTGGCCGTTTCGATC 50.0 50.0 58.0 58.0 2078-2080 bp 1.0 80.7
petA-psbJ_p5 GGGACCGGAACTTCTTGTTT AGTAACTGGCCGTTTCGATC 50.0 50.0 58.0 58.0 1834-1836 bp 1.0 80.7