Hotspot

atpE-atpB

Genome · 104362-104375 · 14 bp

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Pi 0.0703
MarkerSeek score 70.30
Primer available yes
Label atpE-atpB

Feature Metadata

label_name
atpE-atpB
feature_type
igs
region
Genome
length_bp
14
variable_sites
2
pi
0.0703
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpE-atpB_p1 CTGAATCCCAAACGACTCGA GCTACCGCGAAGGCTATTAA 50.0 50.0 57.6 57.9 90 bp 1.0 77.7
atpE-atpB_p2 TCTGAATCCCAAACGACTCG GCTACCGCGAAGGCTATTAA 50.0 50.0 57.6 57.9 91 bp 1.0 77.7
atpE-atpB_p3 CTGAATCCCAAACGACTCGA ATTGATGAAGCTACCGCGAA 50.0 45.0 57.6 57.7 99 bp 1.0 76.2
atpE-atpB_p4 TCTGAATCCCAAACGACTCG ATTGATGAAGCTACCGCGAA 50.0 45.0 57.6 57.7 100 bp 1.0 76.2
atpE-atpB_p5 CTGAATCCCAAACGACTCGA TTGATGAAGCTACCGCGAAG 50.0 50.0 57.6 58.4 98 bp 1.0 75.8