Hotspot

trnS-GCU-trnT-GGU

Genome · 99923-101096 · 1174 bp

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Pi 0.0071
MarkerSeek score 63.10
Primer available yes
Label trnS-GCU-trnT-GGU

Feature Metadata

label_name
trnS-GCU-trnT-GGU
feature_type
igs
region
Genome
length_bp
1174
variable_sites
22
pi
0.0071
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnT-GGU_p1 ATTAGCAATCCGCCGCTTTA GAACCGATGACTTACGCCTT 45.0 50.0 58.0 58.0 1267-1269 bp 1.0 81.2
trnS-GCU-trnT-GGU_p2 ATTAGCAATCCGCCGCTTTA CCATGGCGTTACTCTACCAC 45.0 55.0 58.0 58.1 1246-1248 bp 1.0 80.7
trnS-GCU-trnT-GGU_p3 ATTAGCAATCCGCCGCTTTA TGGCGTTACTCTACCACTGA 45.0 50.0 58.0 58.1 1243-1245 bp 1.0 80.5
trnS-GCU-trnT-GGU_p4 ATTAGCAATCCGCCGCTTTA CCGATGACTTACGCCTTACC 45.0 55.0 58.0 58.2 1264-1266 bp 1.0 80.0
trnS-GCU-trnT-GGU_p5 TAGTGACCGAACGGAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 1326-1328 bp 1.0 79.3