Hotspot

ycf1

SSC · 117410-121195 · 3786 bp

Back to Lappula

Pi 0.00406

MarkerSeek score 34.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3786
variable_sites: 116
pi: 0.00406
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTTTTGTCTGCTCTGCTGC	CTATACGTGCTCAAAGGCGT	50.0	50.0	58.0	58.1	202 bp	0.973684	81.9
ycf1_p2	CTTTTTGTCTGCTCTGCTGC	TACGTGCTCAAAGGCGTAAA	50.0	45.0	58.0	57.8	199 bp	0.973684	81.4
ycf1_p3	GTCTGCTCTGCTGCATACTT	CTATACGTGCTCAAAGGCGT	50.0	50.0	58.0	58.1	196 bp	0.921053	79.7
ycf1_p4	GTCTGCTCTGCTGCATACTT	TACGTGCTCAAAGGCGTAAA	50.0	45.0	58.0	57.8	193 bp	0.921053	79.2
ycf1_p5	GTCTGCTCTGCTGCATACTT	CCGATTTTCGACGAGGCATA	50.0	50.0	58.0	58.2	226 bp	0.921053	79.0