Hotspot

ndhF

SSC · 104580-106793 · 2214 bp

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Pi 0.0028

MarkerSeek score 48.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 52
pi: 0.0028
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGCGAAGATTCAGATAAGCA	TCTTTCAAAGATCCGGCGAA	40.0	45.0	54.6	57.5	2330-2509 bp	1.0	52.2
ndhF_p2	AGCGAAGATTCAGATAAGCA	TTCTTTCAAAGATCCGGCGA	40.0	45.0	54.6	57.5	2331-2510 bp	1.0	52.2
ndhF_p3	AGCGAAGATTCAGATAAGCA	TTCAAAGATCCGGCGAATCG	40.0	50.0	54.6	58.7	2327-2506 bp	1.0	50.2
ndhF_p4	AGCGAAGATTCAGATAAGCA	CTTCTTTCAAAGATCCGGCG	40.0	50.0	54.6	57.2	2332-2511 bp	1.0	49.4
ndhF_p5	AGCGAAGATTCAGATAAGCAT	TCTTTCAAAGATCCGGCGAA	38.1	45.0	55.2	57.5	2330-2509 bp	1.0	49.1