Hotspot

ndhA

SSC · 113431-115546 · 2116 bp

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Pi 0.00336

MarkerSeek score 46.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2116
variable_sites: 56
pi: 0.00336
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCTCTTGTTTGAGAGAAGTTGTGA	TGGGAGAAGTTGATCGTTGA	37.5	45.0	58.0	56.5	2112-2221 bp	1.0	45.8
ndhA_p2	TCTCTTGTTTGAGAGAAGTTGTGA	TGGGAGAAGTTGATCGTTGAA	37.5	42.9	58.0	57.2	2112-2221 bp	1.0	45.8
ndhA_p3	CTCTTGTTTGAGAGAAGTTGTGA	TGGGAGAAGTTGATCGTTGA	39.1	45.0	56.7	56.5	2111-2220 bp	1.0	45.8
ndhA_p4	TCTCTTGTTTGAGAGAAGTTGTG	TGGGAGAAGTTGATCGTTGA	39.1	45.0	56.7	56.5	2112-2221 bp	1.0	45.8
ndhA_p5	TCTTGTTTGAGAGAAGTTGTGA	TGGGAGAAGTTGATCGTTGA	36.4	45.0	55.5	56.5	2110-2219 bp	1.0	45.8