Hotspot

ndhE-ndhG

SSC · 111792-112008 · 217 bp

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Pi 0.00737

MarkerSeek score 51.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 217
variable_sites: 13
pi: 0.00737
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCCAGACACATAAGGGCTCT	AGGTGCAATTGCTGTAGCTC	50.0	50.0	57.7	58.5	320-373 bp	1.0	80.5
ndhE-ndhG_p2	TCCAGACACATAAGGGCTCT	GCAATTGCTGTAGCTCGTCA	50.0	50.0	57.7	58.9	316-369 bp	1.0	77.5
ndhE-ndhG_p3	TCCAGACACATAAGGGCTCT	TGCAATTGCTGTAGCTCGTC	50.0	50.0	57.7	58.9	317-370 bp	1.0	77.5
ndhE-ndhG_p4	AGTTCCAGACACATAAGGGC	AGGTGCAATTGCTGTAGCTC	50.0	50.0	57.2	58.5	323-376 bp	1.0	76.2
ndhE-ndhG_p5	GTTCCAGACACATAAGGGCT	AGGTGCAATTGCTGTAGCTC	50.0	50.0	57.2	58.5	322-375 bp	1.0	76.2