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ndhF-rpl32

SSC · 106794-107381 · 588 bp

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Pi 0.00247

MarkerSeek score 51.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 588
variable_sites: 10
pi: 0.00247
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CAAGGGTATGATCCACGCAT	TGTCCAATACCCCCTCCTTT	50.0	50.0	57.7	57.9	707-742 bp	0.947368	80.1
ndhF-rpl32_p2	AAAAGAAGAAGCCCCACTCC	TGTCCAATACCCCCTCCTTT	50.0	50.0	57.7	57.9	754-789 bp	0.947368	79.7
ndhF-rpl32_p3	AAGCAAGGGTATGATCCACG	TGTCCAATACCCCCTCCTTT	50.0	50.0	57.7	57.9	710-745 bp	0.947368	79.5
ndhF-rpl32_p4	AAGAAGAAGCCCCACTCCTA	TGTCCAATACCCCCTCCTTT	50.0	50.0	57.7	57.9	752-787 bp	0.947368	79.3
ndhF-rpl32_p5	CAAGGGTATGATCCACGCAT	GATGTCCAATACCCCCTCCT	50.0	55.0	57.7	58.2	709-744 bp	0.921053	74.4