Hotspot

psbB-psbT

LSC · 69446-69613 · 168 bp

Back to Lappula

Pi 0.00611

MarkerSeek score 51.90

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 168
variable_sites: 11
pi: 0.00611
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GGAGCATTCCAGAAACTTGGA	TGTAGGCGGTTCTCGAAAAA	47.6	45.0	58.2	57.5	302-318 bp	1.0	71.6
psbB-psbT_p2	GGAGCATTCCAGAAACTTGGA	TTGTAGGCGGTTCTCGAAAA	47.6	45.0	58.2	57.5	303-319 bp	1.0	71.6
psbB-psbT_p3	GGAGCATTCCAGAAACTTGG	TGTAGGCGGTTCTCGAAAAA	50.0	45.0	56.7	57.5	302-318 bp	1.0	70.8
psbB-psbT_p4	GGAGCATTCCAGAAACTTGG	TTGTAGGCGGTTCTCGAAAA	50.0	45.0	56.7	57.5	303-319 bp	1.0	70.8
psbB-psbT_p5	GGTGCTAGAACCTTGTTCAGA	TGTAGGCGGTTCTCGAAAAA	47.6	45.0	57.6	57.5	371-387 bp	1.0	69.3