Hotspot

psbE-petL

LSC · 60721-61729 · 1009 bp

Back to Lappula

Pi 0.00225

MarkerSeek score 52.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1009
variable_sites: 24
pi: 0.00225
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1053-1077 bp	1.0	84.5
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1055-1079 bp	1.0	84.5
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	GCCGCTAGTAGAAAACCGAA	55.0	50.0	58.2	58.0	1048-1072 bp	1.0	84.2
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AAGCCGCTAGTAGAAAACCG	55.0	50.0	58.2	58.0	1050-1074 bp	1.0	84.2
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.4	58.0	1046-1070 bp	1.0	83.0