Hotspot

petA-psbJ

LSC · 59385-59938 · 554 bp

Back to Lappula

Pi 0.00624

MarkerSeek score 57.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 18
pi: 0.00624
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	646-697 bp	1.0	82.0
petA-psbJ_p2	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	661-712 bp	1.0	81.7
petA-psbJ_p3	ACCCATTACGTGTTCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.5	57.6	663-714 bp	1.0	79.4
petA-psbJ_p4	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	656-707 bp	1.0	78.9
petA-psbJ_p5	CGTGTTCAAGGCCTTTTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	655-706 bp	1.0	78.5