Hotspot

rbcL-psaI

LSC · 54384-55775 · 1392 bp

Back to Lappula

Pi 0.00414

MarkerSeek score 42.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1392
variable_sites: 41
pi: 0.00414
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TCGAGGAAGTGGATAAATTGGA	GCCATTGAAATTGCCGGAAA	40.9	45.0	56.9	57.9	1416-1541 bp	1.0	60.7
rbcL-psaI_p2	TCGAGGAAGTGGATAAATTGGA	GAAGCCATTGAAATTGCCGG	40.9	50.0	56.9	58.4	1419-1544 bp	1.0	58.5
rbcL-psaI_p3	TTCGAGGAAGTGGATAAATTGGA	GCCATTGAAATTGCCGGAAA	39.1	45.0	57.6	57.9	1417-1542 bp	1.0	58.1
rbcL-psaI_p4	TCGAGGAAGTGGATAAATTGGA	GCCGGAAATACTAGGCCTAC	40.9	55.0	56.9	57.5	1404-1529 bp	1.0	57.8
rbcL-psaI_p5	CGAGGAAGTGGATAAATTGGA	GCCATTGAAATTGCCGGAAA	42.9	45.0	55.4	57.9	1415-1540 bp	1.0	56.6