Hotspot

ndhC-trnV-UAC

LSC · 48784-49237 · 454 bp

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Pi 0.00575
MarkerSeek score 51.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
454
variable_sites
25
pi
0.00575
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CGGGGCTAAAACTCCTGAAA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.8 58.1 589-727 bp 1.0 84.2
ndhC-trnV-UAC_p2 CCTTTGCTAATCGGGGCTAA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.7 58.1 600-738 bp 1.0 82.9
ndhC-trnV-UAC_p3 ATCGGGGCTAAAACTCCTGA CCGAGAAAGTCTACGGTTCG 50.0 55.0 58.4 58.1 591-729 bp 1.0 82.4
ndhC-trnV-UAC_p4 AATCGGGGCTAAAACTCCTG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.6 58.1 592-730 bp 1.0 82.4
ndhC-trnV-UAC_p5 CGGGGCTAAAACTCCTGAAA AGTGCATGTTGGGTCTTTGA 50.0 45.0 57.8 57.6 653-791 bp 1.0 81.7