Hotspot

ycf3-trnS-GGA

LSC · 43074-43942 · 869 bp

Back to Lappula

Pi 0.00468

MarkerSeek score 52.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 869
variable_sites: 29
pi: 0.00468
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	565-975 bp	1.0	77.4
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TCGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	57.9	586-996 bp	1.0	77.0
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	536-946 bp	1.0	76.7
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	566-976 bp	1.0	75.1
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	GGTTGTTCGGAAAGAGAGGG	47.6	55.0	58.0	58.2	592-1002 bp	0.973684	74.1