Hotspot

rpoB-trnC-GCA

LSC · 26168-27374 · 1207 bp

Back to Lappula

Pi 0.00223

MarkerSeek score 51.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1207
variable_sites: 22
pi: 0.00223
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCCATTACCGAGCA	CCGGATTTGAACTGGGGAAA	47.6	50.0	58.1	58.1	1264-1303 bp	1.0	76.0
rpoB-trnC-GCA_p2	CCTCATTTCCATTACCGAGCA	AAAAGGATTTGCAGTCCCCC	47.6	50.0	58.1	58.4	1246-1285 bp	1.0	73.9
rpoB-trnC-GCA_p3	CCTCATTTCCATTACCGAGC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.3	58.1	1264-1303 bp	1.0	71.0
rpoB-trnC-GCA_p4	CCTCATTTCCATTACCGAGCA	TTTGTTGATCAGGCGACACC	47.6	50.0	58.1	58.8	1283-1322 bp	1.0	70.6
rpoB-trnC-GCA_p5	CCCTCATTTCCATTACCGAGC	CCGGATTTGAACTGGGGAAA	52.4	50.0	58.8	58.1	1265-1304 bp	1.0	70.5