Hotspot

trnS-GCU-trnG-UCC

LSC · 7939-8474 · 536 bp

Back to Lappula

Pi 0.00302

MarkerSeek score 51.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 536
variable_sites: 17
pi: 0.00302
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGACCCT	45.0	42.9	58.0	57.5	480-837 bp	1.0	73.5
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCGAAAGACCCT	50.0	42.9	58.2	57.5	529-886 bp	1.0	72.5
trnS-GCU-trnG-UCC_p3	ACAACAACGGAAAGAGAGGG	TGAATCAAACCGAAAGACCCT	50.0	42.9	57.7	57.5	535-892 bp	1.0	71.7
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAAAGACCCT	50.0	42.9	57.6	57.5	466-823 bp	1.0	70.6
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	TGAATCAAACCGAAAGACCCT	55.0	42.9	58.5	57.5	461-818 bp	1.0	69.8