Hotspot

clpP

LSC · 68850-70814 · 1965 bp

Back to Laportea

Pi 0.0594

MarkerSeek score 83.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1965
variable_sites: 337
pi: 0.0594
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	ACGAACATACCCTGTCTCCA	50.0	50.0	58.0	58.1	2837-2914 bp	1.0	91.3
clpP_p2	CTGAACGAGTCGCACATACA	CGTATAGAGCCATCGAACCG	50.0	55.0	58.0	58.0	2773-2849 bp	0.8	83.5
clpP_p3	ATGATCTGAACGAGTCGCAC	CGTATAGAGCCATCGAACCG	50.0	55.0	58.1	58.0	2778-2854 bp	0.8	83.0
clpP_p4	GATCTGAACGAGTCGCACAT	CGTATAGAGCCATCGAACCG	50.0	55.0	58.1	58.0	2776-2852 bp	0.8	83.0
clpP_p5	ACATGATCTGAACGAGTCGC	CGTATAGAGCCATCGAACCG	50.0	55.0	58.1	58.0	2780-2856 bp	0.4	65.0