Hotspot

atpF

LSC · 10993-12298 · 1306 bp

Back to Laportea

Pi 0.0535

MarkerSeek score 84.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1306
variable_sites: 196
pi: 0.0535
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ATACGAGCAATACCATCGCC	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	57.9	58.0	2110-2177 bp	0.8	83.1
atpF_p2	CTGAAATTTCACCCCGACCA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.1	58.0	2401-2422 bp	0.7	78.9
atpF_p3	AGCAATTCCTACCGTACCCT	TAATTGGGGGAGGGGGAATT	50.0	50.0	58.1	58.0	1868-1877 bp	0.2	51.5
atpF_p4	CTGAAATTTCACCCCGACCA	TAATTGGGGGAGGGGGAATT	50.0	50.0	58.1	58.0	2046-2055 bp	0.2	51.4
atpF_p5	ATACGAGCAATACCATCGCC	TAATTGGGGGAGGGGGAATT	50.0	50.0	57.9	58.0	1810 bp	0.1	43.2