Hotspot

rpl20-rps12

LSC · 67773-68582 · 810 bp

Back to Laportea

Pi 0.0652

MarkerSeek score 87.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 142
pi: 0.0652
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 87.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTGGTTCTACGTCTTCGAGC	CAGCGACGAGGAACATGTAC	50.0	55.0	57.7	58.7	837-899 bp	1.0	83.4
rpl20-rps12_p2	TTGGTTCTACGTCTTCGAGC	TCAGCGACGAGGAACATGTA	50.0	50.0	57.7	58.8	838-900 bp	1.0	82.6
rpl20-rps12_p3	TGGTTCTACGTCTTCGAGCT	CAGCGACGAGGAACATGTAC	50.0	55.0	58.5	58.7	836-898 bp	1.0	82.4
rpl20-rps12_p4	TTGGTTCTACGTCTTCGAGC	AGCGACGAGGAACATGTACT	50.0	50.0	57.7	58.8	836-898 bp	0.9	78.7
rpl20-rps12_p5	TTGGTTCTACGTCTTCGAGC	GCGACGAGGAACATGTACTA	50.0	50.0	57.7	57.2	835-897 bp	0.8	74.5