Hotspot

trnC-GCA-petN

LSC · 27006-27906 · 901 bp

Back to Laportea

Pi 0.101

MarkerSeek score 89.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 251
pi: 0.101
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 89.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	904-1007 bp	1.0	90.1
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	965-1068 bp	1.0	88.5
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	922-1025 bp	1.0	87.9
trnC-GCA-petN_p4	GGGTGTCGCCTGATGATAAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.7	58.2	948-989 bp	0.4	63.3
trnC-GCA-petN_p5	GGGTGTCGCCTGATGATAAA	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.7	57.6	1009-1050 bp	0.4	61.3