Hotspot

rps16-trnQ-UUG

LSC · 5683-6107 · 425 bp

Back to Laportea

Pi 0.228
MarkerSeek score 75.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
425
variable_sites
199
pi
0.228
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.88
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 490-739 bp 0.8 77.1
rps16-trnQ-UUG_p2 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 493-742 bp 0.8 74.9
rps16-trnQ-UUG_p3 CGTTGCTTTCTACCACATCG TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.2 57.3 494-743 bp 0.8 72.4
rps16-trnQ-UUG_p4 CACGTTGCTTTCTACCACATC GAGGTTCGAATCCTTCCGTC 47.6 55.0 57.5 58.1 492-741 bp 0.8 71.7
rps16-trnQ-UUG_p5 CACGTTGCTTTCTACCACAT GAGGTTCGAATCCTTCCGTC 45.0 55.0 56.4 58.1 492-741 bp 0.8 70.3