Hotspot

petD

LSC · 77039-78266 · 1228 bp

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Pi 0
MarkerSeek score 63.60
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1228
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 AGTTGAAGGGAATTCTCCGA CCCTAGATACACATGTCGGG 45.0 55.0 56.2 56.9 1246-1307 bp 1.0 60.2
petD_p2 AGTTGAAGGGAATTCTCCGA GCTTTGAAGCGACTATTCCC 45.0 50.0 56.2 56.9 1263-1324 bp 1.0 60.1
petD_p3 TGAAGGGAATTCTCCGAAGAG CCCTAGATACACATGTCGGG 47.6 55.0 57.1 56.9 1243-1304 bp 1.0 59.8
petD_p4 TGAAGGGAATTCTCCGAAGAG GCTTTGAAGCGACTATTCCC 47.6 50.0 57.1 56.9 1260-1321 bp 1.0 59.7
petD_p5 AGTTGAAGGGAATTCTCCGAAG CCCTAGATACACATGTCGGG 45.5 55.0 58.1 56.9 1246-1307 bp 1.0 58.5