Hotspot

petB

LSC · 75464-76833 · 1370 bp

Back to Lancea

Pi 0.0212
MarkerSeek score 70.90
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1370
variable_sites
29
pi
0.0212
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 TTGGTAGTTCGATCGTGGAA TCCTACTATTCCTCCTCTAAGTGA 45.0 41.7 56.6 57.2 1477-1490 bp 1.0 41.6
petB_p2 TTTGGTAGTTCGATCGTGGA TCCTACTATTCCTCCTCTAAGTGA 45.0 41.7 56.6 57.2 1478-1491 bp 1.0 41.6
petB_p3 TGGTAGTTCGATCGTGGAAT TCCTACTATTCCTCCTCTAAGTGA 45.0 41.7 56.4 57.2 1476-1489 bp 1.0 41.6
petB_p4 AGAACTCGGATTTTGGCCTA TCCTACTATTCCTCCTCTAAGTGA 45.0 41.7 56.5 57.2 1504-1517 bp 1.0 41.5
petB_p5 TAGAACTCGGATTTTGGCCT TCCTACTATTCCTCCTCTAAGTGA 45.0 41.7 56.5 57.2 1505-1518 bp 1.0 41.5