Hotspot

trnV-GAC-rps7

IRa · 138153-140652 · 2500 bp

Back to Lancea

Pi 0.00569
MarkerSeek score 61.50
Primer available yes
Label trnV-GAC-rps7

Feature Metadata

label_name
trnV-GAC-rps7
feature_type
igs
region
IRa
length_bp
2500
variable_sites
14
pi
0.00569
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps7_p1 GATGACTTCCACCACGTCAA TTCTTCTGCAGTACCTCGAC 50.0 50.0 57.8 57.0 2539-2577 bp 1.0 72.3
trnV-GAC-rps7_p2 AACTGATGACTTCCACCACG TTCTTCTGCAGTACCTCGAC 50.0 50.0 57.8 57.0 2543-2581 bp 1.0 72.2
trnV-GAC-rps7_p3 ATGACTTCCACCACGTCAAG TTCTTCTGCAGTACCTCGAC 50.0 50.0 57.8 57.0 2538-2576 bp 1.0 72.2
trnV-GAC-rps7_p4 CTGATGACTTCCACCACGTC TTCTTCTGCAGTACCTCGAC 55.0 50.0 58.3 57.0 2541-2579 bp 1.0 71.3
trnV-GAC-rps7_p5 GATGACTTCCACCACGTCAA TCTTCTGCAGTACCTCGACG 50.0 55.0 57.8 59.2 2538-2576 bp 1.0 70.8