Hotspot

rps12-trnV-GAC

IRb · 98829-100481 · 1653 bp

Back to Lancea

Pi 0.00868
MarkerSeek score 65.70
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1653
variable_sites
14
pi
0.00868
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1711-1749 bp 1.0 79.1
rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1715-1753 bp 1.0 79.1
rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1710-1748 bp 1.0 79.1
rps12-trnV-GAC_p4 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1713-1751 bp 1.0 78.2
rps12-trnV-GAC_p5 TGGCAACTTTACGTAAGGCA GTCAAGGTGACACTCTACCG 45.0 55.0 57.7 57.7 1696-1734 bp 1.0 77.7