Hotspot

rpl36-infA

LSC · 80191-80285 · 95 bp

Back to Lancea

Pi 0.0211
MarkerSeek score 64.60
Primer available yes
Label rpl36-infA

Feature Metadata

label_name
rpl36-infA
feature_type
igs
region
LSC
length_bp
95
variable_sites
2
pi
0.0211
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-infA_p1 ATTACTATAATGCGCCCCCG TATGATTCAACCAGAGGGCG 50.0 50.0 57.6 57.4 232 bp 1.0 72.6
rpl36-infA_p2 ATTACTATAATGCGCCCCCG GATTCAACCAGAGGGCGTAT 50.0 50.0 57.6 57.4 229 bp 1.0 72.5
rpl36-infA_p3 ATTACTATAATGCGCCCCCG ACGGATACTGCCAGGAGATA 50.0 50.0 57.6 57.3 275 bp 1.0 72.0
rpl36-infA_p4 ATTACTATAATGCGCCCCCG ATACGGATACTGCCAGGAGA 50.0 50.0 57.6 57.3 277 bp 1.0 72.0
rpl36-infA_p5 ATTACTATAATGCGCCCCCG TACGGATACTGCCAGGAGAT 50.0 50.0 57.6 57.3 276 bp 1.0 72.0