Hotspot

rpl20-rps12

LSC · 69563-70368 · 806 bp

Back to Lancea

Pi 0.0211
MarkerSeek score 71.10
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
806
variable_sites
17
pi
0.0211
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCCGAGCTATATATCCGCGT ATGTGACAAAATCCCCTGCA 50.0 45.0 58.0 57.7 905-910 bp 1.0 78.9
rpl20-rps12_p2 CAGTCTTGAATGAGCCCCTC ATGTGACAAAATCCCCTGCA 55.0 45.0 58.0 57.7 964-969 bp 1.0 78.8
rpl20-rps12_p3 TCCGAGCTATATATCCGCGT GTGACAAAATCCCCTGCACT 50.0 50.0 58.0 58.4 903-908 bp 1.0 78.4
rpl20-rps12_p4 TCCGAGCTATATATCCGCGT TGACAAAATCCCCTGCACTT 50.0 45.0 58.0 57.6 902-907 bp 1.0 78.4
rpl20-rps12_p5 CAGTCTTGAATGAGCCCCTC GTGACAAAATCCCCTGCACT 55.0 50.0 58.0 58.4 962-967 bp 1.0 78.3