Hotspot

ycf4-cemA

LSC · 61034-61940 · 907 bp

Back to Lancea

Pi 0.00342
MarkerSeek score 64.00
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
907
variable_sites
3
pi
0.00342
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGGCCTATTTCTTGCGTGT GAGAGAGAAATCCACCAGGG 45.0 55.0 58.1 57.0 992-1023 bp 1.0 73.9
ycf4-cemA_p2 GAACAAAAAGCCGCGGAATT GAGAGAGAAATCCACCAGGG 45.0 55.0 58.2 57.0 1010-1041 bp 1.0 72.9
ycf4-cemA_p3 AACAAAAAGCCGCGGAATTG GAGAGAGAAATCCACCAGGG 45.0 55.0 58.5 57.0 1009-1040 bp 1.0 70.6
ycf4-cemA_p4 AATTGAACAAAAAGCCGCGG GAGAGAGAAATCCACCAGGG 45.0 55.0 58.5 57.0 1014-1045 bp 1.0 70.6
ycf4-cemA_p5 CGCGGAATTGGCCTATTTCT GAGAGAGAAATCCACCAGGG 50.0 55.0 58.7 57.0 999-1030 bp 1.0 69.2