Hotspot

psbZ-trnG-GCC

LSC · 36182-36484 · 303 bp

Back to Lancea

Pi 0.0133
MarkerSeek score 60.80
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
303
variable_sites
4
pi
0.0133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CCGGTACATCGTTGTGGATT AATCGAACCCGCATCTTCTC 50.0 50.0 58.0 58.1 426-430 bp 1.0 80.5
psbZ-trnG-GCC_p2 CCGGTACATCGTTGTGGATT GAATCGAACCCGCATCTTCT 50.0 50.0 58.0 58.1 427-431 bp 1.0 80.5
psbZ-trnG-GCC_p3 TTTCCGGTACATCGTTGTGG AATCGAACCCGCATCTTCTC 50.0 50.0 58.2 58.1 429-433 bp 1.0 79.0
psbZ-trnG-GCC_p4 TTTCCGGTACATCGTTGTGG GAATCGAACCCGCATCTTCT 50.0 50.0 58.2 58.1 430-434 bp 1.0 79.0
psbZ-trnG-GCC_p5 TTGCTTCTCCTGATGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 471-475 bp 1.0 78.9