Hotspot

trnS-GCT-trnR-TCT

LSC · 8042-9758 · 1717 bp

Back to Lancea

Pi 0.00932
MarkerSeek score 56.30
Primer available yes
Label trnS-GCT-trnR-TCT

Feature Metadata

label_name
trnS-GCT-trnR-TCT
feature_type
igs
region
LSC
length_bp
1717
variable_sites
16
pi
0.00932
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCT-trnR-TCT_p1 CAATCCGACGCTTTAGTCCA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1788-1789 bp 1.0 64.3
trnS-GCT-trnR-TCT_p2 AATCCGACGCTTTAGTCCAC AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1787-1788 bp 1.0 64.3
trnS-GCT-trnR-TCT_p3 CAATCCGACGCTTTAGTCCA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1794-1795 bp 1.0 63.6
trnS-GCT-trnR-TCT_p4 AATCCGACGCTTTAGTCCAC AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1793-1794 bp 1.0 63.6
trnS-GCT-trnR-TCT_p5 ACGGAAAGAGAGGGATTCGA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.2 56.9 1842-1843 bp 1.0 63.1