Hotspot

psbI-trnS-GCT

LSC · 7836-7953 · 118 bp

Back to Lancea

Pi 0.0169
MarkerSeek score 62.20
Primer available yes
Label psbI-trnS-GCT

Feature Metadata

label_name
psbI-trnS-GCT
feature_type
igs
region
LSC
length_bp
118
variable_sites
2
pi
0.0169
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCT_p1 ATCTAATGATCCGGGGCGTA TGGACTAAAGCGTCGGATTG 50.0 50.0 58.1 58.0 231 bp 1.0 80.1
psbI-trnS-GCT_p2 TATCTAATGATCCGGGGCGT TGGACTAAAGCGTCGGATTG 50.0 50.0 58.1 58.0 232 bp 1.0 80.1
psbI-trnS-GCT_p3 ATCTAATGATCCGGGGCGTA GTGGACTAAAGCGTCGGATT 50.0 50.0 58.1 58.0 232 bp 1.0 80.1
psbI-trnS-GCT_p4 TATCTAATGATCCGGGGCGT GTGGACTAAAGCGTCGGATT 50.0 50.0 58.1 58.0 233 bp 1.0 80.1
psbI-trnS-GCT_p5 ATCTAATGATCCGGGGCGTA ATTGGGAGAGATGGCTGAGT 50.0 50.0 58.1 58.1 250 bp 1.0 79.2