Hotspot

matK-rps16

LSC · 3493-4513 · 1021 bp

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Pi 0.0127
MarkerSeek score 66.20
Primer available yes
Label matK-rps16

Feature Metadata

label_name
matK-rps16
feature_type
igs
region
LSC
length_bp
1021
variable_sites
13
pi
0.0127
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-rps16_p1 CCACGATCATGAGCAAATGC GTGCTCAACCCACAGGAAC 50.0 57.9 57.9 58.7 1216-1217 bp 1.0 66.6
matK-rps16_p2 ACAACCACGATCATGAGCAA GTGCTCAACCCACAGGAAC 45.0 57.9 57.8 58.7 1220-1221 bp 1.0 66.1
matK-rps16_p3 CCACGATCATGAGCAAATGC TGCTCAACCCACAGGAACT 50.0 52.6 57.9 58.8 1215-1216 bp 1.0 65.8
matK-rps16_p4 ACAACCACGATCATGAGCAA TGCTCAACCCACAGGAACT 45.0 52.6 57.8 58.8 1219-1220 bp 1.0 65.3
matK-rps16_p5 CCACGATCATGAGCAAATGC TCCTTGAAAAAGGTGCTCAAC 50.0 42.9 57.9 56.9 1228-1229 bp 1.0 63.4